Sentence examples for transformants and of from inspiring English sources

This number was significantly higher than that of mock transformants and of previous studies using similar plasmids [ 34, 35].

A β-glucan plate assay was used for preliminary evaluation of enzyme production by the transformants and for selection of representative clones from the transformation for further analysis.

P. pallidum cells were transformed by electroporation with the linearized vector pAcgA-KO, followed by selection of transformants and diagnosis of gene knock-out as outlined in Supplementary figure 3.

pRF4 contains rol-6 su1006), rol-6 su1006 rolling phenotype, allowing deteconferringransforollingand maintenance of transgenic lines.

Further crossing of G0 flies, screening of the transformants and balancing of insertions performed at the Transgenic Fly Facility at C-CAMP facility at NCBS campus, Bangalore, India.

Frozen stocks of single transformants and pools of transformants were prepared by standard methods.

Finally, both methods require identification of positive E. coli transformants and purification of the plasmid for further use.

A total of 150 transformants were analyzed for PCR products as pools (Figure 3B), each pool containing ten transformants, and one of the pools generated a PCR product of appropriate size.

The applied methodology, tested in two well-known strains, allows the production of large numbers of transformants and the construction of large recombinant libraries.

Recombinant Bombyx mori nucleopolyhedrovirus (rBmNPV-NcSAG1) bacmids were extracted from white transformants and insertion of the NcSAG1 gene was confirmed by PCR.

Deletion of this gene results in uracil auxotrophy and resistance to 5-fluoroorotic acid (5-FOA), allowing prototrophic selection of transformants and counter-selection of the wild-type allele [ 22].