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We obtained 44 co-transformants, and identified co-transformant L 61, which showed the highest laccase activity among all the transformants.

We obtained 44 co-transformants, and identified co-transformant L 61, which showed the highest laccase activity among all of the screened transformants.

The recombinant plasmid was transformed into E. coli JM109, and transformants were identified by restriction digest analysis and sequencing.

Arabidopsis transformation was performed by the flora-dip procedure [ 40] and transformants were identified by screening for kanamycin resistance.

Positive transformants were identified and isolated using a Q-Pix automated colony picker.

The constructs were injected into w 1118 embryos and transformants were identified on the basis of eye color.

Additional β-lactamase genes carried in parental strains and transformants were identified by using the Check-MDR CT101 microarray assay (23 ) (Table 4).

More then 20 transformants were identified and 15 transformants were tested for the presence of the basta resistance gene, the transgenic old3-1 gene and the wild type OLD3 gene.

A total of four independent transformants were identified and in all cases most neurons were positive for QF as assessed by multiple lines bearing QUAS-PA-GFP; we used the line with the highest expression levels.

AD and BD plasmids were transformed into PJ69-4 [ 36] and dual transformants (identified as colonies growing on media lacking leucine and tryptophan, the selective markers for these two plasmids) subsequently tested on media lacking leucine, tryptophan, and adenine (the latter being one of the scorable markers for interactions).

These effector constructs were directly transformed into the transgenic GUS reporter plants and double transformants were identified through screening for antibiotic (hygromycin) resistance.

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