Sentence examples for transformants and further from inspiring English sources
Clones yielding colonies were considered as positive transformants and further checked for correct integration of the respective deletion cassette.
Analyses to determine MmTKL1 expression in the transformant and further, putative third gene will be needed.
Further studies should therefore be carried out to determine the safety of this C. rosea transformant and further assess its capacity to control soybean Sclerotinia stem rot.
Competent E. coli BL21 DE3) cells (Invitrogen, Carlsbad CA), which have isopropythio-β-D-galactoside (IPTG -inducible T7 RNA polymerase expressIPTG -inducibleformed wiT7 pJL100 oRNAJL200; some of these transformants were made compolymerase furthexpressionrmed were pJL300.
These strains were screened for growth and lipid contents based on optical density and Nile red fluorescence, respectively, and we selected NsbHLH2 3–6 and 3 11 transformants for further analyses (Additional file 3: Figure S3, Additional file 6: Text S1).
These pTUM4 transformants were further transformed with pASK-IBA32 plasmid encoding the Bj-xtrIT(His6) gene and plated on agar with 100 μg/ml AMP plus 25 μg/ml CAM overnight at 37 °C.
These transformants were further cultured and purified using HisTrap Ni Sepharose.
Lines with successful transformants were further propagated and progeny were screened for loss of coinjection mCherry fluorescence markers but continued rescue of the Unc phenotype, indicating successful integration of the construct.
The transformants were selected on the solid plate and further cultured in the liquid medium of YES supplemented with G418 and glucosamine.
Southern analysis confirmed the deletion (shift from >10 kb for the WT to a double band at 3.2 and 3.0 kb for Δ tku70 strains B14 and C146), and further showed that both transformants had undergone a single integration event (Fig. 1c).
Isolated transformants were further tested by PCR#A and PCR B, resulting in 22, 19, and 22 integrated strains (Table 1, HBG 1-3).
