Sentence examples for transformants all of from inspiring English sources

Although with differences among individual transformants, all of them were more virulent than the xyn11A mutant N23 and close to the wild-type strain B05.10.

This level of ampicillin is high enough to select for the plasmid and consistently gave BL21 (DE3) transformants with all of our pDEP plasmids.

Construction of orc1/whip Mutants of S. islandicus Generation of pLRMG transformants All pLRMG plasmids were linearized by restriction digestion and 0.9-1μg linearized plasmid DNA was used to transform the genetic host S. islandicus E233S by electroporation as described previously (Deng et al., 2009 ).

Of the 41 constructs, eight plasmids pSYMP-Rv06811, -phoY2, -hspX, -ino1, -leuD, -fadE24, -tsf, and -cmaA2) resulted in undetectable recombinant protein or very few transformants of all three Mtb test strains, although TAP/MS had previously shown that three of the test proteins (HspX, Ino1, Tsf) were pupylated in the RCC pupylome (Table 1).

The best transformants of each of the gene combinations were chosen.

A 96-well plate activity screening for transformants of all constructs was established and the best expressing clone was used for large-scale production in 50-L scale, which gave a volumetric yield of 223 mg L−1 PDH or 1,330 U L−1 d−1 in space time yield.

Arginine auxotrophs were found in one of 32 transformants of pyrG13 (targeting frequency of 3%) and in 29 of 31 transformants of MR12 (94%).

In all transformants, PCR fragments of the expected sizes were amplified and, thus, the genomic integration of the artificial gene constructs could be confirmed by PCR.

All transformants of ATCC 824 and the wild type displayed higher solvent production levels when grown without pH-regulation.

All transformants gave colonies of comparable size on glucose plates.

Transformation efficiencies were expressed as the number of transformants per micrograms of plasmid DNA.