Sentence examples for transformants after the from inspiring English sources

Once you obtain bacterial transformants after the second round, pick at least 50 colonies, recover plasmids individually and sequence.

To create double transformants, after the CPBF1-silenced strain was established, they were further cultivated without G418 for at least 4 weeks to remove the silencing plasmids, and G418 sensitivity was examined.

For selection of transformants, after electro-pulse the recovery cultures with pDCW89 DNA (0.5 – 1.0 μg) were plated onto the defined medium without casein and uracil.

Three out of eight transformants survived after the antibiotic treatment and were used for further analysis.

Ninety percent of the transformants obtained after the induction of I- SceI were stable against 44 and 58%% of colonies grown under repressed conditions and for the control strain, respectively (Table 3).

The frequency of LOH for total Ura+ transformants (after selection for loss of the CF) from the mus81 Δ mutant was not significantly less than observed for wild type, and chromosomal recombinants were recovered at a similar frequency to wild type.

The transformants, after being centrifuged and washed twice with 1 mL of deionized distilled water, were selected on the SC-URA plate at 30°C until URA+ colonies appeared.

Here, a loss in enzyme activity and CGP accumulation occurred in transformants after several cultivation rounds, whereas the gene itself remained present in the cells (Dr. A. Bröker, personal communication).

The total recombinant GBSS-parasite fusion protein content was determined in all transgenic transformants after starch purification.

The swimming transformants were re-grown in the same conditions used in Figure 6, and probed 0 h and 48 h (CYC6 transformants) or 0 h and 6 h (CAH1 transformants) after induction.

The primers used for this construction are listed in Table S2. Figure S6 shows the expression of HPT-1-FLAG in the control P hpt-1hpt-1 strasn, as well as in several P tcu-1hpt-1 primary transformants after growth in 250 µM CuSO4 and 200 µM BCS.