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Specifically, a P. stipitis genomic DNA library transformed into a transporter-null strain of S. cerevisiae yielded no transformants able to grow on xylose [ 64].

Two transformants able to grow on supplemented glucose minimal medium were revealed, one from each group of molecular masses (Fig. 4).

Transformants able to grow at 38° were colony purified and replica-plated to YPD medium containing 100 μg/mL nourseothricin.

It is noteworthy that despite several attempts, transformation experiments done with the s1 mutant as a recipient did not yield any transformants able to express the Secteur.

None of the AML1 fragments cloned into pGADT7, and cotransformed with empty pGBKT7 control vector, yielded transformants able to grow on selective medium.

Homologous integration by double crossover will generate transformants able to use acetamide as the sole nitrogen source (due to the presence of the amdS gene) but unable to produce any penicillins.

We obtained approximately 36000 independent transformants of which approximately 500 transformants were able to grow on the galactose-containing plates.

The mstG transformants were able to grow on glucose, galactose, mannose and sucrose as single carbon sources, whereas mstH transformants grew on glucose, sucrose, mannose, galactose and, in contrast to mstG strains also on fructose.

The results indicate that C. reinhardtii transformants were able to produce 63 and 94% more neutral lipids than the wild-type, which translates to an approximately 56% improvement in total lipids, without compromising growth.

Following isolation and transfer of hygromycin-resistant mycelial slices to media containing increasing concentrations of hygromycin B, we found that transformants were able to propagate in the presence of hygromycin B concentrations as high as 300 μg/ml (Figure 3c, bottom panel).

All of the transformants were able to grow on YM plates containing 4.5 mM 5-FOA, indicating that the URA3 reporter gene in the prey plasmid was not expressed.