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The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV SPT15-EcoRI-FW/SPT15-SalI-RV
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We then transformed these six genes and a selection marker gene into the KY3 genome using PGASO.
However, tissues transformed with these genes and/or with those encoding for desirable traits could harbour both transformed and non-transformed cells.
Several plant species including Arabidopsis, tobacco, poplar, rice, Eastern cottonwood, peanut, salt marsh grass and Chlorella have been transformed with these genes.
We also chose not to log transform the gene expression data.
We used the exponential function y = exp(- x) to transform the gene-gene distance to gene-gene similarity.
In particular, we use a preliminary E. coli TRN and transform the gene-gene scores to gene-TF scores.
BAC clone CHORI-242-264H20 contAKR1C2AKR1C4, AKR1C4, the urocortin 3 (UCN3), tubulin alpha-like 3 (TUBand) aneuroepithelialial cell transforming gene 1 (NET1) genes.
We fused 1.1 kb of the upstream regions containing the putative promoter of these genes to reporter genes and transformed these into the Indian malaria vector A. stephensi to see if we could recapitulate the expression pattern of the endogenous genes.
Loss of function mutations in any of the BX-C genes transforms these respective segments toward more anterior identity (Celniker et al., 1990; Duncan, 1987; Sánchez-Herrero et al., 1985).
Another interpretation is that, because only the early SV40 genes are necessary to transform cells, only these genes are expected to be expressed in mesotheliomas (Carbone et al., 2008).
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