The strains were made prototrophic by transforming sequentially the CEN.PK2-1C strain with (i) the required expression cassettes for LEU2, TRP1 or HIS3, (ii) the GPD1 and/or GPD2 deletion cassette and (iii) YEpJK01plasmid.
Heat-induced DY380 cells containing the retrieved DNA segment were transformed sequentially with MTVs which had been excised from the pBluescript vector backbone to generate the final targeting vector.
ROS generation appears to be triggered by the activation of the mitochondrial-dependent cell death pathway through the proapoptotic Bcl-2 proteins Bax and is further transformed sequentially into more toxic ROS, like hydrogen peroxide, which, consequently, induces cell death [ 44].
To uncouple this cycle of response and counter response between plant and insect attackers, we fed larvae on isogenic plants that had been transformed to sequentially silence a suite of JA-mediated defenses (N, TPIs, and all JA-mediated defenses) and analyzed the transcriptional responses in neonates with an unbiased experimental protocol.
In particular, during the hydrogenation and subsequent etching of both the carbon cap and the tube, they sequentially transform to different carbon nanostructures, including carbon nanosheet, nanowall, and polyyne chains, before they are completely removed from the surface of a substrate-bound Ni-nanocluster.
The resveratrol biosynthesis pathway consists of four enzymes that sequentially transform phenylalanine into this specialised secondary metabolite.
These neuroepithelial cells sequentially transform into neuroblasts, which generate differentiated neural cells of the medulla through asymmetric divisions.
Subject's MPRAGE scan was co-registered to MNI template, using affine transformation before sequentially transforming each subject's structural image to the group average, and combining these to construct a new average image.
These two plasmids were sequentially transformed into E. coli, generating a library of ∼5 × 10 transformants.
It explains exact linearization of used, initially nonlinear models, which are sequentially transformed to discrete state-space form.
