An important caveat is that the low CAIs of the transferred genes observed here may be an artifact due to HGT identification by abnormal nucleotide compositions.
Ferredoxin systems are known to improve the metabolic reactions by mediating electron transfer in partner enzymes as observed here [ 27- 29].
This is consistent with a general observation that a structure capable of pausing the RT also facilitates transfer reactions; however, the effect observed here is less striking than for two other G-quadruplex forming sequences in a region of gag and cPPT.
17 This process involves a transient ion-pairing that facilitates the transfer across the interface, which agrees with the phenomenon observed here, although it fails to explain the exclusion of alkali metal cations.
Whether the effect of SVP[Rapa] observed here, in the context of liver gene transfer with AAV8 vector doses that are clinically relevant5,6, will be identical for other capsids or at higher vector doses remains to be established.
Anomalous concentration dependences observed here may be reconciled by considering the role of phase transfer processes that mediate concentrations in each phase.
The primary KIE observed here agrees with previous conclusions that NADH oxidation is stereospecific, transferring the hydride from the B-face (pro-S position) of its nicotinamide ring.
The fact that the polaron signal is still rising beyond 1 ns and is observed here at very high excitation fluences suggests that the hole transfer is inefficient even for short n-butylamine ligands.
Compared to typical nanoimprinting with silica bead arrays in which transfer of the nanoparticles to the imprinted films is often seen, it is not observed here.
The thinning or etching rate observed here is clearly higher than that value, indicating that the oxidation is a charge-transfer (or electrochemically -aided procelectrochemically -aided
