For those sticking with Windows, Detto also makes IntelliMover -- a PC-to-PC transfer kit that can move files, e-mail address books, Internet connection settings, bookmarks and more from one Windows system to another and put everything in the right place.
The blotting step was carried out with the Bio-Rad Trans-Blot®Turbo™ RTA Transfer kit onto a nitrocellulose membrane.
Harvested samples were separated by SDS-PAGE under denaturing conditions and transferred onto nitrocellulose membranes using an iblot transfer kit (Life Technology, Frederick, MD, USA).
Purified ATH35L was fractionated by SDS-PAGE and then transferred onto a nitrocellulose membrane using an iblot transfer kit (Life Technology, Frederick, MD, USA).
Briefly, the 15mer peptide 65-79*0404 was labeled with Sulfo-SBED in an equal molar ratio, using the ProFound™ Sulfo-SBED Biotin Label Transfer Kit (Thermo Scientific).
To examine the functional relevance of MC-derived Gal-1, we adoptively transferred Kit W-sh/W-sh mice with Gal-1-deficient (Lgals1 −/− ) BMMCs.
After electrophoresis, the proteins were electroblotted onto nitrocellulose membranes (Amersham Bioscience) at 0.8 mA cm-2 for 1 h using the semi-dry transfer kit (Bio-Rad).
Our results indicate that exosomes can transfer KIT as a protein to tumor cells, which can affect recipient cell signaling events through receptor-ligand interactions.
By chance, several items of date-expired stock were identified in the transfer kit whilst moving a patient to a tertiary centre.
Furthermore, HMC-1 exosomes contain and transfer KIT protein, but not the c-KIT mRNA to A549 cells and subsequently activate KIT-SCF signal transduction, which increase cyclin D1 expression and accelerate the proliferation in the human lung adenocarcinoma cells.
