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Transferring the entry library from pDONR222 to the Y2H destination library (pDEST22) was done in a single tube recombination reaction using LR Clonase™ II following the manufacturer's instructions (Invitrogen).
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How does it work? A. This approach to filling prescriptions transfers the data entry, insurance adjudication, follow-up with doctors' offices and other administrative work out to a central processing facility.
A recombination (LR) reaction is then performed which allows to transfer the cDNA from this entry vector to the destination vector (pBS-RfA).
Rather, income transfers are the entry point to large scale integrated national responses to children and families affected by HIV and AIDS.
The IDS's electronic databases captured all diagnoses during this time period; for diagnoses recorded on paper, data entry clerks transferred the information into the databases.
Two data entry specialists transferred the data into EpiData, a data management program; each double-entering the data to minimize miscoding.
A data entry officer transferred the completed CCD Observation Forms into The Census and Survey Processing System (CSPro), a public domain software package developed and supported by the U.S. Census Bureau and ICF Macro, which is interfaced with Statistical Package for the Social Sciences SPSSS 12.0).
We transferred the data, using a double-entry procedure to ensure its integrity, from the returned questionnaires into a database for analysis.
The sorptive properties of EPS effectively chelate toxic metals and other contaminants, which then act as an efficient trophic-transfer vehicle for the entry of contaminants into food webs.
In the experiments that utilized splenectomized mice to ascertain liver specific priming of CD8+ T cells, mice were treated with Mel-14 Ab (100 µg/mouse) by IP administration one day before adoptive transfer to limit the entry of Thy1.1+OT-1+CD8+ T cells into the LNs.
Transferring all data entries in an organized form to the ZENODO platform makes them easily accessible.
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CEO of Professional Science Editing for Scientists @ prosciediting.com