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Inoculations were performed a minimum of 3 days after transferring plants to reduce plant stress from transfer.
The high-temperature treatment was imposed by transferring plants at anthesis to 38°C for 1 day.
Inoue et al. (2003) exposed very young seedlings to Fe-sufficient nutrient solution for 2 weeks, whereas Kobayashi et al. (2009) cultivated seedlings in Fe-sufficient nutrient solution for 3 weeks, before transferring plants to Fe-deficient solution for 1 week.
Simply transferring plants from one control media to another also resulted in decreased DNA methylation (Figure 6D).
Flowering was induced by transferring plants to growth chamber maintained at 22°C with long day condition (16-hour-day and 8-hour-night cycles).
Stress treatments were done for 24 hours by transferring plants to 500 mM mannitol supplemented media for osmotic stress, or by incubating them at 37°C.
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Don't reuse seed-raising mix; that risks transferring plant diseases that can decimate your seedlings.
In some places, the growth of the unregulated businesses continues to raise questions of fairness -- particularly where utilities have been permitted to transfer plants to the new units at deep discounts to their market value.
This approach allows us to see transferred plants as active agents in region-forming processes, and to avoid normative tropes like 'miracle plants' or 'alien invasives'.
One week after transfer, plants were inoculated with S. meliloti strain 2011 (OD600 = 0.05-0.1).
Genetic engineering involves breaking the DNA barrier to directly transfer plant and animal genetic material.
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