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RNA samples (1 µg in 38 µL nuclease free water) were combined with 2 µL Oligo-dT anchored primers (Life Technologies, 12577-011) and incubated at 80°C for 2 minutes before transferring immediately to ice for 2 minutes.
Using 0.5 μg of single-stranded circular M13mp19 phage DNA and standard annealing buffer (20 mM Tris HCl, pH 7.5, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT) the labeled oligodeoxynucleotide was annealed by heating at 95°C for 1 min, transferring immediately to 65°C for 2 min and then cooling slowly to room temperature.
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Transfer immediately to a bowl.
The cash will not transfer immediately to the Irish revenue.
Drain and transfer immediately to the cold water.
Transfer immediately to a bowl of ice water, then drain.
Some disappointed passengers were transferred immediately to another Premier boat, or got another cruise.
Using a skimmer or slotted spoon, transfer immediately to the ice water.
The killers hid the baby dolphin under a tarp and transferred immediately to Taiji Whale Museum.
The school did, however, lose four teacher's aides, who had to be transferred immediately to other schools.
After a few moments, the first infusion is poured into the slender and taller cup, then transferred immediately to what looks like a sake cup or shot glass.
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