Subsequently, 100 µL of fresh complete medium was added to the virus-antibody mix and 140 µL of the virus-antibody mix was transferred back to the cells after the old cell medium was discarded.
After incubation, serum and virus suspensions were transferred back to Vero cell monolayer containing plates.
Exactly the same logic would allow the cell producing the mRNA to lose the ability to translate mRNA (e.g., to lose its ribosomes), instead depending on the coupled cell to do so, with the resulting proteins being transferred back to the first cell (the proto-nucleus) via the nanotubes.
These manually assigned labels were transferred back to the single cell level for further processing.
The culture was then incubated for further 15 minutes, after which the cell culture was transferred back to 25°C to allow the cells to recover.
The resulting 2-oxoglutarate is transferred back to the bundle sheath cells.
Lymphocytes obtained from the resolution phase (72 hours) of normal strain-matched wild-type controls which were therefore composed of B1, NK, and γ/δ T cells as well as CD4+/CD25+ cells, were transferred back to gp91phox knockout mice (72 hours) and challenged with LPS.
Interestingly, the reshaping of mitochondria is reversible, the percentage of donut-shaped mitochondria decreased when quiescent TG1 cells are transferred back to pH 7.4 (Fig. 4C).
After 3 days in IL3-negative medium, surviving cells were transferred back to regular growth medium with 0.3 ng/ml IL3 for 3 days.
However, when the cells were transferred back to culture conditions with serum, the enhanced IKBKAP exon 20 inclusion was not maintained (Figure 8G and H).
After heat treatment, A. sinensis suspension cells were transferred back to shaker at 25 °C and 110 rpm until harvested.
