Sentence examples for transferring 1 from inspiring English sources
To evaluate the effect of bottleneck size in the rate of clonal extinction, we performed an additional experiment where 20 lines of T. thermophila, all derived from the same founding clonal population, were grown in the same conditions as the initial MA lines, except instead of transferring 1 single cell every 48 hours we performed daily transferences of 1000 cells.
After transferring 1 g of the sample into the test tube, the suspension was prepared.
Serial dilutions were made by transferring 1 ml of the previous dilution in 9 ml of sterile distilled water up to 1 10,000 dilutions.
It was shaken vigorously to dissolve the soil and form a homogenized solution, followed by subsequent tenfold dilution by transferring 1 ml solution to sterile 9 ml deionized water-containing tubes until a dilution of 10−6 was obtained.
For the TCC, a confirmatory test by transferring 1 ml of the aliquots from each dilution into three tubes of Lauryl sulfate tryptose (LST) broth and incubating at 35 °C for 48 h was also done.
This was done by preparing serial dilutions from 10−1 to 10−6 by transferring 1 ml homogenized sample (1 10 dilution) to 9 ml sterile saline water, mixing well with vigorous shaking and continuing until 10−6 dilution was reached.
Ap were subcultured by transferring 1/50th of an infected culture to a new flask containing sterile host cells.
Chimeras were generated by transferring 1 1 mixed WT Pak2 +/+ (CD45.1+CD45.2+) and KO Pak2 F/F ;Cd4 -Cre (CD45.2+) donor bone marrows into lethally irradiated C57BL6 hosts that express CD45.1.
One culture was synchronized for another 3 days and the other was kept exponentially growing by transferring ∼1 × 10 cells into fresh media and analyzing the remaining cells by FACS every 10 and 14 hr for 3 days.
Worm samples were prepared by transferring 1-day-old adult hermaphrodite worms to 20% bovine serum albumin (BSA) in M9 buffer in the cavity of an aluminum planchette (type 'A' hat; 100 µm deep, Wohlwend, Switzerland) for high-pressure freezing (McDonald et al., 2007).
