The new policy is more effective in encouraging the application of organic fertilizer when the issuing of land certificates is enforced and administrative barriers to land right transfers are removed.
If dinoflagellate gene regulation avoids promoters, and instead relies on trans-splicing and gene dosing, a potentially significant barrier to gene transfer is removed.
Perhaps the worst thing that prison officials attempted to do to me due to my writing was to have me transferred before my cancer was removed.
The supernatant was removed, transferred to a new centrifuge tube and re-centrifuged at 16,000 g for 10 min at 4°C to facilitate removal of the lipid layer overlying the supernatant.
The intact chloroplast layer, identified by microscopy, was removed, transferred to SDS-PAGE cocktail, boiled for 10 min and stored at -20°C.
The aqueous phase was removed, transferred to a separate tube containing 500 μL of isopropanol (Sigma), gently mixed and centrifuged for 10 mins at 12,000 × g at 4 °C.
The spleen of each gibel carp was removed, transferred to 1.5 ml tubes with an aliquot value of RNA later TM Storage Solution (Sigma-Aldrich) and stored at −80°C.
Three milliliters of chloroform were added, mixed well, and the samples centrifuged for 10 min. The organic layer was removed, transferred into a 3-mL glass vial, and left to dry in a fume hood overnight.
The contents were separated by centrifugation at 903×g for 5 minutes, then the chloroform layer was removed, transferred into a pre-weighed tube (tube 3; weight 1) and dried for 30 min under a flow of N2 at 60°C.
In 2008 the UK Border Agency gave birth to the Border Force, which existed as part of the wider agency till March 2012, when it was removed and transferred into the Home Office proper.
