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The transferred vector was checked by DNA sequencing.
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Transient transfections were performed to transfer vectors to B16F1 murine cells.
This strain can be efficiently transformed with a transfer vector containing the desired gene circuit and the corresponding attP site together with a plasmid encoding the corresponding recombinase.
The 220 momentum transfer vector lies orthogonal to the direction of the applied electric field, and therefore provides measurement of crystallographic changes transverse to the applied field direction.
Sleeping Beauty (SB) transposon is a nonviral gene transfer vector, already used in clinical trials.
We have developed a gene transfer vector system based on JDV that has three components.
For recombinase-based transformations, 10 μg of circular transfer vector and 10 μg of circular recombinase expression vector were combined, added to 80 μl of competent cells and incubated for 5 min in an ice-cold 0.2 cm electroporation cuvette.
In this study, the MOMP gene was amplified by PCR and cloned into the transfer vector pShuttle-CMV.
CocH expressed by adenoviral gene transfer vector enables durable and effective levels of enzyme expression in liver and plasma.
For lentivirus production, HEK293T cells were transfected with the transfer vector (e.g., pCDH-CMV-MCS-EF1-Puro or pGIPZ), pCMV-dR8.91 packaging plasmid, and pCMV-VSV-G envelope plasmid in a 5 1 4 ratio using the Calcium Phosphate Transfection Kit (Clontech).
The 333 momentum transfer vector for neutron diffraction is parallel to the direction of the applied electric field, and therefore provides measurement of crystallographic changes parallel to the applied field direction.
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