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Gels were stained prior to transfer to use 18s-rRNA as a loading control for each blot.

In this case, the images have been digitally transferred to canvas using pigment, and again the format is problematic.

John XXIII also arranged for the remains of Pope Pius X to be transferred to Venice using the Vatican railway.

Western blots were carried out by standard methods on proteins transferred to PVDF using TransFi (Invitrogen).

The protein was transferred to membranes using the Invitrogen iBlot® transfer system (catalogue number IB101).

Samples were subjected to reducing SDS PAGE and transferred to nitrocellulose using a semidry blotter.

The videos were transferred to computers using the iMovie 2011™ (Apple Inc., 2011) software and stored.

The samples were then transferred to acetonitrile using a gentle flow of nitrogen.

Following SDS-PAGE, proteins in gels were transferred to nitrocellulose using an iBlot (Life Technologies).

The extract was then transferred to vials using methanol for LC-MS and ELISA analysis.

Proteins were resolved by SDS-PAGE and transferred to nitrocellulose using an iBlot apparatus (Life Technologies).

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