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For experiments that required fasting, mice were transferred to clean cages containing floor grids and food was removed for the indicated time.
Surviving treated larvae were then washed twice in clean distilled water and transferred to clean cups with 98 ml of distilled water and 2 ml of larval food.
The inserts were then transferred to clean wells containing 400 μl of cell staining solution from the kit and were incubated for 10 min at room temperature.
All samples were sent to the laboratory, suspended in 1 mL of 0.1 M PBS (phosphate buffered saline), transferred to clean sterile eppendorf tubes and kept frozen at -20°C until processed.
The rats' livers were then removed and transferred to clean beaker and weighed.
The culture supernatants were transferred to clean flat-bottom plate for enzymatic analysis.
Clearance of each PCB depended also on its initial load and solubility, being faster in clams transferred to clean systems.
Following the uptake period, fish are transferred to clean water to measure the depuration of the test substance.
The supernatants containing thyroid hormones were transferred to clean tubes and dried at 45 °C for 4 h.
Neither CeO2 NPs nor SnO2 NPs bioaccumulated in earthworms, and both were rapidly excreted when worms were transferred to clean soil.
The resulting mixture was centrifuged at 3500 g for 5 minutes at 4°C to remove the suspended matter, with supernatants transferred to clean amber glass tubes for liquid-liquid extraction.
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