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The digested solution was then transferred to a sample vial for LC-MS analysis.
The samples were frozen with liquid nitrogen slush, then transferred to a sample preparation chamber at −160°C and etched for 15 min at −85°C.
Fresh leaves of Begonia guixiensis (Peng et al. 20310) and B. longa (Peng et al. 20076) were dissected and attached to a stub, frozen with liquid nitrogen slush, and then transferred to a sample preparation chamber at −160°C and etched for 15 min at −85°C.
Hundred µl of cell suspension was transferred to a sample cup together with a solution, containing 5 mM palmitate (unlabeled and 0.5 µCi [1-14C] palmitate) (Du-Pont, NEN, specific activity 55.8 mCi/mmol) and 16.7 mM glucose.
The ultrafiltrate was then transferred to a sample cup and analyzed.
After 10 min, the mixture was immediately transferred to a sample chamber of multifunction chemiluminescence detector IFFS-A.
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The resulting clot was first transferred to a sample-embedding capsule and then to a 4% PBS-buffered formalin solution.
The sample was transferred to a square sample pool, and the measurement was repeated three times.
The mixture was transferred to a polyethylene sample cup.
Matrix containing C18-bead bound peptides were transferred to a MALDI sample plate.
An aliquot of 30 μl was transferred to a ZnSe sample carrier (BrukerOptics GmbH, Ettlingen, Germany) and dried for 40 minutes at 40°C.
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