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At the end of the third week, a valgus deformity of the transferred segment of fibula was corrected by realigning the distal Schanz pins under fluoroscopy.
Comparisons of these sequences show that the complete upstream region necessary for the gene's expression is included in the transferred segment.
For example, a horizontally transferred segment can appear as a dark red or black arc in a portion of the map consisting of otherwise weakly similar genomes.
Through operon prediction by DOOR (Database of prOkaryotic OpeRons) [ 65], we found that this large horizontally transferred segment around rpsF was predicted as an operon.
As for the large horizontally transferred segment around mopB, operon prediction indicated that mopB and mopS are also involved in an operon, which implies that they are horizontally transferred together.
The mean length of a HT fragment is about 1300 nucleotides (see Materials and Methods), while the longest transferred segment consists of more than 7000 nucleotides, and contains 9 HT Na-ATPase genes.
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Spliceosomal-type introns were found to be inserted in four HGT genes, while non-coding regions of two large horizontally transferred segments were lacked on the shrimp genome.
As information about horizontally transferred segments is available only for a subset of our initial data set, for this analysis we used 316 genomes, which resulted in 393 learning examples.
There are numerous data types users may wish to visualize on CCT maps, such as gene expression measurements, the positions of horizontally transferred segments revealed by specialized programs, the positions of SNPs identified by sequencing, and so on.
The horizontally transferred large segment around rpsF was observed to exclude non-coding regions in the shrimp genome.
This technology, commercially termed Gateway™ (Invitrogen), bypasses the need for traditional ligase-mediated cloning, provides high specificity and activity, while maintaining orientation of the transferred DNA segment and yielding a high proportion of desired clones [ 28- 32].
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