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At hour 16, rinse plates that hold transferred round cells with PBS (30ml/plate).
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In a typical procedure, Ce(NO3 3·6H2O was dissolved in double-distilled water and the resulting clear solution was transferred to round bottom flask.
Liu et al. [69] calculate the page transferred at round i, v_{i} = begin{cases} v_{mem} quad qquad text{if} quad mathrm{i=0} d*t_{i-1} qquad text{otherwise} end{cases} (3).
Cells were transferred into round bottom polypropylene tubes (Becton Dickinson).
To explore NCAM localisation, tumour cells were transferred to round cover slips, which were placed in a 24-well multiplate.
To explore NCAM localization, tumor cells were transferred to round cover slips which were placed in a 24 well multiplate.
According to these corresponding groups, solutions were transferred to round bottom flasks and shaked on orbit shaker (BioTechnics, India) for mixing.
The filtered cell suspension was concentrated by centrifugation at 200 g for five minutes and the cells subsequently resuspended in 5 ml PBS/1%HSA and transferred to round bottom tubes for incubation with antibody-coated beads.
To analyze integrin beta1 distribution on the cell membrane, tumor cells were transferred to round cover slips (pretreated with 2% 3-aminopropyl-triethoxysilan) placed in a 24 well multiplate.
MDCK cell monolayers were trypsinized and transferred to round plastic cover slips placed in 24 well styrene plates and kept at 37°C in 5% CO2; after 24 h cells formed confluent monolayers.
Mouse melanocytes grown on 24-mm glass coverslips were transferred to round adaptors (OKO-lab) with 2 ml of medium supplemented with 25 m m HEPES, pH 7.4, and placed on a heated stage at 37°C, to be observed in bright field either with a PlanApochromat 63× n.a.a
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