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20 μl of cell lysate was transferred into one frame on the slide surface and incubated for 2 h at room temperature.
Twenty vitrified/warmed blastocysts were surgically transferred into one uterine horn.
The oil was then recovered in a stainless-steel container and transferred into one litre transparent glass jars.
The solid accumulated at bottom of beaker was separated and transferred into one flat-bottom flask and the same 10 ml solution inside beaker was added to it.
Concomitantly, 500 μl of samples was also transferred into one isotype control and three sample tubes and the appropriate antibodies were added: Then, 9 ml of ACK lysing buffer was added (to lyse red blood cells), vortexed briefly and incubated at room temperature (18 25 °C) for 3 min.
The cells were transferred into one single tube, followed by full panel antibodies cocktail staining and DNA intercalator staining.
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The nutrient mediums were transferred into one-fourth volume of the plate.
Cervical swabs were collected using a sterile flocked swabs (Copan, Italy) and transferred into one-tube DNA purification system (OTP Reagent, Bioron, Germany) container.
All the images are treated by using the ACDSee image treatment software, the colorful ones transferred into gray ones, non-BMP images transferred into BMP ones, and all of them are cut into sizes of.
After germination, uniform seedlings transferred into aerated one-half strength Hoagland solution for 3 days.
The 5-days-old seedlings were transferred into the one-half strength modified Hoagland solution with or without iron supply, and cultivated for 3 days.
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