Patients who had transferred in to care on ART were excluded in this analysis since baseline HIV RNA viral loads were not known.
The resulting colonies were transferred in replica on two plates: one containing kanamycin and ampicillin and another containing sucrose and ampicilin.
After one hour of incubation 100 μL of the supernatants was transferred in triplicates on a 96-well plate and absorbance was measured at 555 nm on a Wallac 1420 VICTOR2 plate reader (PerkinElmer, Waltham, MA, USA).
Nuclear and cytosolic extracts were prepared according to Yerneni et al. 1999 [31]. 2 µg total protein of nuclear or cytosolic extracts were transferred in duplicates on nitrocellulose membrane by Dot Blotting (Dot Blot 96 System, Biometra, Goettingen, Germany).
They excluded files belonging to patients who transferred in while on ART from another facility after January 2010 in order to avoid double counting had they also been sampled from the facility where they initiated ART.
Images were transferred in DICOM format on CD and subsequently analysed using OsiriX Lite.
One or two best quality blastocysts were transferred in the uterus on day 5.
The house was transferred in pieces and reconstructed on the CSM roof terrace in September 2015.
But I'm gonna show him that I'm not gonna let this go". .
