Sentence examples for transferred in duplicate from inspiring English sources
1.0×106 cells in 100 µl of media were transferred in duplicate wells to a 96-well plate.
Aortic rings were then transferred in duplicate to glass scintillation vials containing 200 µl of lucigenin (5 µM), which was previously dark adapted for 30 min. The chemiluminescence was recorded every minute for 10 min at room temperature using a liquid scintillation counter (Wallac 1409; Perkin Elmer Life Science, St Laurent, Quebec, Canada).
Sera diluted 1 : 100 in BB were transferred in duplicate to the ELISA plates.
After centrifugation supernatants were transferred in duplicate to a 96-well plate and extinctions were measured at 570 nm.
A 7 μL culture from each well was transferred in duplicate to 384-well plates, in which cell lysis, Firefly, and Renilla measurements were carried out.
Total cell lysates from HeLa SS6 cells transiently transfected with the indicated GFP-tagged FCHO1 protein fusions were resolved by SDS-PAGE and transferred in duplicate to nitrocellulose.
Nuclear and cytosolic extracts were prepared according to Yerneni et al. 1999 [31]. 2 µg total protein of nuclear or cytosolic extracts were transferred in duplicates on nitrocellulose membrane by Dot Blotting (Dot Blot 96 System, Biometra, Goettingen, Germany).
After 1 hour of incubation, 100 µL of each serum virus solution was transferred in duplicates to the wells with the cells.
The amplified PCR products were transferred in duplicates to nylon membranes (Hybond-N, Amersham Pharmacia Biotech, UK) in a 3 × 3 pattern leaving an empty area in the middle for local background subtraction.
Twenty-five μl was transferred to duplicate wells of a microtiter plate and snap-frozen in liquid nitrogen.
The procedure was repeated until all samples were transferred to individual wells in duplicate.
