The technology transfers flow from Russia's lucrative contracts to supply Iran with a nuclear power reactor and advanced conventional weapons.
(2) Suppose that pump chamber is connected to transfer flow through at least one cone-shaped tube.
Via P-gp activity transfers, flows of cells depopulate and repopulate resistant and sensitive cells.
Samples were transferred to flow cytometry tubes, and cells were counted using a FACSCalibur™ (BD – USA) flow cytometer.
Consequently, the cells were resuspended with 500 μL PBS (pH 7.4) and transferred into flow cell tubes.
Cells were then suspended in staining buffer and transferred for flow cytometry (BD LSR II, BD biosciences).
Cells were pelleted, washed twice, resuspended in ∼300 µl of FACS staining buffer and transferred to flow cytometry tubes for cytometric analysis [33].
To confirm the functionality of the transferred cells, flow cytometric analysis was performed with splenocytes of reconstituted mice at days 10 and 15 p.i.
The cells were then trypsinized, resuspended in full growth medium, and transferred to flow cytometry tubes.
Cells were transferred to flow cytometry tubes and stained with 3.3 μl of PI (50 μg/mL).
Cells were washed twice with PBS, detached with 100 μL accutase (PAA; Pasching, Austria) and transferred into flow cytometry tubes.
