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Rentzsch and Orengo [ 38] transferred annotations in single profile-based sequence cluster.
However, the great majority (∼95%) of GO corresponds to automatically transferred annotations based on InterProScan (Quevillon et al., 2005) results.
Validation of the transferred annotations showed their high reliability, which makes them suitable to be used for data-driven biomedical knowledge discoveries.
In a final step, protein domains, families and motifs are identified through InterProScan and the corresponding GO terms are merged with the already transferred annotations taking care of redundancies and GO hierarchy.
In order to evaluate the reliability of the transferred annotations we analysed the potential effect of detected variants projecting them on the corresponding protein coding sequences in each variety.
Several (19.31 %) of the transferred gene to pathway annotations involve Reactome pathways, although such transferred annotations concern only human genes (since only for Homo sapiens Reactome provides protein annotations not computationally inferred).
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Among newly assigned functions, some have been provided definite names (Table 8) and this transferred annotation information should be unquestionable.
We downloaded the latest TAIR [ 59] Gene Ontology annotation file and transferred annotation to Populus and O. sativa trough the orthologs.
> -wrap-foot> In Table 2, we address how much of the transferred annotation is also endowed with an experimental evidence code.
We have therefore designed and implemented a novel tool, Genome Annotation Transfer Utility (GATU), to transfer annotations from a previously annotated reference genome to a new target genome, thereby greatly reducing this laborious task.
For example, the subcellular localization data gives a spatial cellular landscape of whole genome proteins within a cell, while the orthologous information can help to annotate unknown genes by transferring annotations between orthologs.
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