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Mouse A9 cells used as a fusion recipient for chromosome transfer were maintained at 37°C in Dulbecco's modified Eagle's medium (DMEM; Sigma, St Louis, MO, USA) plus 10% FBS.
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In order to examine whether or not bilateral tool transfer was maintained in a short-term reciprocal manner, we tested the mothers and offspring in a situation in which they could not expect any short-term reciprocation from the partner.
The network of liaison officers on the Programme Against African Trypanosomiasis (PAAT), through training, information dissemination and technology transfer, was maintained.
The valve oven and transfer lines were maintained at 325°C.
The injector and transfer line were maintained at 225°C and 250°C respectively.
The GC injector and transfer line were maintained at 280 °C and 250 °C, respectively.
To avoid condensation of the reaction products, all transfer lines were maintained at a constant temperature of 180 °C.
The ion source, quadrupole and transfer line temperatures were maintained at 230°C, 200°C and 200°C, respectively.
The ion trap (250°C), manifold (280°C), and transfer line (280°C) were maintained at constant temperatures.
The silenced plants did not survive after transfer to soil, and were maintained on the high-sucrose media continuously.
The cultures were maintained without transfer for several weeks.
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