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In order to fully demonstrate suspected cases of cps region horizontal transfer, we sequenced in 177 relevant isolates, a 360-nt internal portion of gene gnd, which genomic location is just adjacent of the cps operon [50], [51].
To gain insight into the pathomechanisms and the underlying genetic elements for lateral gene transfer, we sequenced the entire genome of this pathogen.
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Since oriT is an essential element for mediating horizontal gene transfer we searched for a similar sequence in pPDL2.
In this study, in order to assess the possibility of lateral gene transfer, we determined the full-length sequences of these transcripts, and performed detailed structural and phylogenetic analyses.
To determine if a complete bacteriophage WO transfer occurred between coinfections, we sequenced the genome of the B- Wolbachia infection of the parasitic wasp Nasonia vitripennis (wVitB) and compared it with the prophage sequences of the A- Wolbachia (wVitA) that coinfects the same host.
At this point (56 transfers, 27 hours of growth), we sequenced an isolate and found three novel mutations (C878T, G2085C, and C2179T).
To see if the original sequences are horizontal transfers, we examined two horizontal transfer databases: HGT-DB [ 56] and HGT Analysis Database [ 57].
In multiple genome assembly, such nodes are not necessarily repeats since they can sometimes be shared between the genomes of two closely related species and represent orthologous sequences, conserved sequences (such as rRNA sequences) or horizontal transfer sequences.
In multiple genome assembly, such node is not necessarily a repeat since it is sometimes shared between the genomes of two closely related species and represents orthologous sequences, conserved sequences (such as rRNA sequences) or horizontal transfer sequences.
Chimeric nodes are shared between the genomes of two closely related species and could represent orthologous sequences, conserved sequences (e.g. rRNA sequences) or horizontal transfer sequences.
The whole P1-2A and 3C coding regions in transfer plasmid was sequenced and named pVL-P12A3C.
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