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At 4 weeks after CD4+ T-cell adoptive transfer, we detected donor CD3+CD4+ T cells in VAT, SAT and spleen (Fig 6B).
Besides that, using fluorescence resonance energy transfer we detected mispaired TCR dimers and different pairing behaviors of individual TCR chains with a mutual influence on TCR chain expression.
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To further explore how it improves the development of porcine nuclear transfer embryos, we detected the global acetylation levels of Histone 3 at Lysine 9 (AcHistoneHistone 3 at Lysine 18 (and3K18), and Histone 4 at Lysine 16 (AcH4K16) using immunofluorescence in the CBHA treated embryos and control embryos.
In order to examine the effects of non-Treg CD25+ cells used in adoptive transfer experiments, we detected the percentages of CD4+Foxp3− T-cell population in each group, and there was no statistic differences between CHS+Ag and TN+Ag group (data not shown).
In addition to decreased mutant SERPINA1 polymer, upon TFEB gene transfer, we also detected a significant reduction of SERPINA1 mRNA and monomer.
We detected transfer of 12 CHCs and putative triacylglycerides after copulation (Figure 5, Table 5) with significant amounts of seven CHC components including four putative triacylglycerides and C46 and C48 hydrocarbons, both of the latter containing O2 groups.
We detected transfer partners for 1,682 genes among those 812 gene families.
However, upon transfer to LIF-Stat3, we detected an extremely rapid and efficient activation of the reporter, with GFP-positive cells appearing as early as day 2 and with an efficiency of reporter activation of 5% after 6 days in LIF-Stat3.
In the current study we detected transferred cells surviving and proliferating to 30 days, but the absolute number of recovered cells is small and may reflect a diminishing survival with time.
We detected intermolecular transfer of energy via imaging FRET and observed by electrophoresis in native conditions that MEGF10 destabilized the oligomeric assemblies of the ABCA1 transporter.
Using this approach, we detected significant transfer to our African American sample for 7 of 84 (8.3%) testable loci (Supplementary Table S1).
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