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In vitro fertilization embryo transfer was performed within 3 months after surgery.
A simulation of heat, charge, and mass transfer was performed to obtain effective transport properties.
In this study, the heat transfer was performed with a constant heat flux applied on the top and bottom surfaces of the minichannel heat exchanger.
Gene transfer was performed from either the intimal surface (Group I, n = 18) or the adventitial surface (Group II, n = 18).
A 3D CFD approach, based on transient conjugate heat transfer was performed on a regenerator sector to obtain the fluid flow and heat transfer characteristics of each configuration.
Transfer was performed at 35 V ON.
Retrovirus-mediated gene transfer was performed as previously described [44].
Serial blood transfer was performed at 2 weeks post-inoculation, during the expected time of peak viremia (Fig. 4A).
The transfer was performed in a transfer buffer (25 mM Tris base, 0.192 M glycine, PH 8.3) at 30 V for 2 h.
Electrophoresis was performed using an XCell SureLock Mini-Cell (Invitrogen Corp .. Protein transfer was performed wet with a Mini Trans-blot Cell (Bio-Rad, Hercules, California, United States).
Confirmation of plasmid transfer was performed by pulsed-field gel electrophoresis (PFGE) of nondigested and digested DNA of the transconjugant, donor and recipient strains.
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