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Information on patient mortality and clinic transfer was collected by personal information and telephone calls with contact persons and from the hospital wards.
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Emission signals from a donor excited with a 532 nm DPSS laser (Cobalt, 100 mW) and an acceptor excited by energy transfer were collected and recorded using EM-CCD (Andor iXonEM+897), with lab-developed imaging software and a 50 ms time resolution.
One limitation is that only a relatively limited number of data items about transfer were collected.
Although data on the reasons for transfer were collected, there were no explicit data on the urgency of transfer.
Additional data on team decision making about appropriateness for rehabilitation, and readiness for transfer, were collected on a subset of patients.
Patient characteristics (gender, year of birth, type of hospital admission, number of days in hospital prior to ICU transfer, number of prescribed medication before hospital admission, and Acute Physiology and Chronic Health Evaluation II) (35) at the moment of transfer were collected using Open Clinica (36).
The mycelia from the transfer cultures was collected by filtration, then frozen and ground in liquid nitrogen.
Two and four days after the transfer, shoot tissue was collected from the seedlings.
Transferred cellular material was collected from the tubes and STR profiles generated using the AmpFlSTR SGM Plus™ multiplex with 28 and 34 PCR cycles.
Tail muscle was collected, transferred into liquid nitrogen, and stored at -80°C for later analysis.
The resulting supernatant was collected, transferred into a new centrifuge tube, and centrifuged again to recover any cells that were not collected in the primary pellet.
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