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A synthetic chaperone consisting of the granzyme B ER signal peptide and a domain encompassing putative N-linked glycosylation sites in granzyme B is insufficient for payload transfer to target cells, whereas full-length granzyme B is sufficient for payload delivery.
We found that the high aspect ratio of the immunological synapse was insufficient in this regard, and that molecular crowding within the synapse is critical to preserve sufficient concentrations of perforin and granzyme for consistent pore formation and granzyme transfer to target cells.
As elsewhere, the N-linked glycan is assembled on phosphodolichol carriers before transfer to target Asn residues.
PRO 2000 present during capture of the replication-defective HIV JRFL) reporter virus or replication-competent HIV(BaL) by monocyte-derived DC (MDDC) inhibited subsequent HIV transfer to target cells.
They reported that Ad modified by bi-functional antibodies could improve gene transfer to target cells in vitro [ 17– 17].
Cullin neddylation increases CRL ubiquitylation activity via conformational changes that optimize ubiquitin transfer to target proteins (Duda et al., 2008).
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It can be easily harvested from the original growth substrate and be transferred to target substrates.
Therefore we introduced 5 measure times: t0 - agent gets created, t1 - agent gets started at the Agent Management VM, t2 - agent loaded its configuration, t3 - agent got transferred to target VM, t4 - agent starts working on target VM.
Graphene meshes were transferred to target substrates using a poly methylmethacrylate) (PMMA) protecting layer according to a method described elsewhere [5, 30], followed by thermal annealing in vacuum to minimize the contamination from residual polymers.
The post-copy technique is effective when the majority of pages are transferred to target server before page faulty occur at destination VM and minor page faults occur due to network faults.
The highly expressed miRNAs in MVs were transferred to target cells after MV incorporation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com