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To induce callusing from explants, cultures were exposed to low- (4 °C) and high-temperature (33 °C) treatments for 0 or 15 days prior to their transfer to standard culture room conditions.
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After completion of our standardized discharge criteria, patients were transferred to standard care units for further recovery.
Slices were transferred to standard ACSF (in mM: NaCl 130, KCl 2.5, NaH2PO4 1.25, NaHCO3 25, dextrose 10) and kept at 34°C until use.
Newly eclosed flies that had been raised on standard food were transferred to standard food supplemented with 100 mM L-NAME or D-NAME for 48 h before being subject to AL or STS feeding regimens.
The locations were transferred to standard sections taken from a brain atlas [ 15].
Remaining larvae were transferred to standard cornmeal media without Edu and allowed to develop until wandering third instar stage.
Injected worms were individually transferred to standard NGM plates at room temperature and allowed to exhaust the food source.
The cells were transferred to standard culture medium after 24 h, whereupon they were used for experiments.
After 24 hours, cells were rinsed with PBS and transferred to standard medium with 10% FBS or to medium without serum.
L1 animals of the bhEx31 strain were transferred to standard NG agar plates containing Escherichia coli OP50 bacteria and grown for a desired period of time.
Gametocytes were Nycodenz purified from infected blood and either immediately fixed (unactivated) or transferred to standard ookinete culture medium for activation and fixed at 8 mpa.
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