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A synthetic pathway for production of the eukaryotic trimannosyl chitobiose glycan (mannose3-N-acetylglucosamine2, Man3GlcNAc2) and its transfer to specific asparagine residues in target proteins was previously engineered in Escherichia coli, providing this simple microbe with the ability to perform a complex post-translational protein modification.
For example, mitochondrial Zn pools in neurons accumulate and are later mobilized in response to exogenous or endogenous cues [24] prior to transfer to specific cytosolic Zn pools [25].
It involves the assembly of glycans on a lipid carrier in the ER membrane, followed by a transfer to specific asparagine residues of target polypeptides [ 19].
The key charged amino acids are adjacent to the triad tryptophans, suggesting that UV-B excitation of tryptophans could result in proton-coupled electron transfer to specific charged amino acids, neutralizing interactions that maintain the dimer [ 2, 12, 14, 15].
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In those cases, the horses are transferred to specific pastures owned by the bureau for an easy retirement.
We exemplarily showed how this framework could be transferred to specific tasks and item responses which subsequently led to appropriate measurement models (Kuo and Wu 2013).
It implies that policy makers can alter the intra-household allocation of resources by targeting taxes or transfers to specific household members.
Tiny drops (10 20 µm) of the cell suspension were quickly transferred to specific areas of either 2D or 3D culture systems.
Ten to twelve week-old germ-free mice were transferred to specific pathogen free (SPF) conditions (conventionalized), and after 5 weeks were injected i.p. with 10 mg/kg body weight AOM (Sigma Aldrich) once a week for 6 weeks.
Arabidopsis (Arabidopsis thaliana) seeds were surface sterilized and sown on Murashige and Skoog plates, then kept at 4°C in darkness for 3 to 5 days, and transferred to specific light conditions at 22°C.
After quantification DNA will be diluted in buffer Te (0.1 mM EDTA, 10 mM Tris HCL, pH 8.0) till a final concentration of 100 n/μl and then transferred to specific tubes identified (ScreenMates 1.4 mL Storage Tubes – Matrix Technologies Corp Ltd).
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