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For profiling of the immune response induced by immuno-flap transfer, the expression of cytokines, such as IL-2, IL-4, IL-10, 1L-17A, IFN-γ, Foxp3 and NKR-P1A, was analyzed using RT-PCR.
At 1, 2 and 4 weeks after the transfer, the expression of IL-7 mRNA and protein in the BM, spleen and colon of recipient mice was determined (figure 4A).
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Transgenic plants were generated by Agrobacterium-mediated transfer with the expression of both cDNAs being under the control of the tuber specific B33 patatin promoter.
We have found that p21cip1 gene transfer downregulates the expression of various inflammatory mediators and tissue-degrading proteinases that are critically involved in the pathology of RA.
A survey of the literature shows that miRNA-142 is upregulated in neurons and its overexpression through stable gene transfer downregulates the expression of NAD-dependent deacetylase Sirtuin 1 (SIRT1) gene and consequently the expression of the monoamine oxidase (MAOA) gene [ 13].
A woman's iron status, for example, is associated with her iron metabolism genotype and may also impact placental lead transfer by influencing the expression of metal transport machinery that is common to both iron and lead.
Local transfer of MCs restored normal number of implantations at sites of MC transfer and augmented the expression of Mcpt1, Mcpt5 and Mcpt8 on mRNA levels (Supplementary Figure 3 and Supplementary Figures 4a c and Table 2).
GJIC in the cells was determined using the scrape loading and dye transfer (SLDT) assay; the expression of Cx43 was detected by RT-PCR, immunofluorescence and Western blot analysis.
Transcriptional analyses showed that SERCA2a gene transfer differentially induced the expression of a subset of genes and appears, in general, to reverse the transcriptional profile induced by diabetes.
We examined the efficiency of peptide-mediated gene transfer by evaluating the expression of the complexed EGFP gene.
In a novel viral-mediated gene transfer rat model, the expression of D620N VPS35 induces the marked degeneration of substantia nigra dopaminergic neurons and axonal pathology, a cardinal pathological hallmark of PD.
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