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Reversible addition-fragmentation chain transfer self-condensing vinyl polymerizatransfer self-condensingrsatile methodology for synthesizing hyperbranched polymers with a large number of chain transfer termini.
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The fact that the recipient construct can be cleavage-deficient strongly argues against the reconstitution of active monomers by transfer of N termini to 7TM domains in a split personality receptor scenario, as in our assay the inactive N terminus at the recipient receptor was inaccessible for replacement through lack of GPS cleavage.
Differing from previous studies, terminus transfer occurred at a short distance from the invasion site.
Introduction of structure into the acceptor template shifted the location of terminus transfer.
Restriction sites within the region of homology allowed efficient mapping of the location of primer terminus transfer.
Overall results support that the acceptor structure has a major influence on the efficiency and position of the invasion and terminus transfer steps.
Our recent studies suggest that the process can occur through a multi-step mechanism involving RNase H cleavage, acceptor invasion, branch migration, and finally primer terminus transfer.
Reading is a major junction point of the National Rail system, and hence Reading station is a major transfer point and terminus.
The crystal structure of MtfA complexed with (S -adenosyl-L-methionine, (S -adenosyl-L-methionine or S -adenosyl-L-methionineupled with mutageneS -adenosyl-L-methionineS -adenosylhomocysteine required for methyl tranS -adenosylhomocysteineor the glycopeptide.
They contain a RING finger domain at their NH2-terminus and transfer ubiquitin-chains to the proteins destined for proteasomal degradation [ 158, 162].
The optimization of the dianionic form, with both negatively charged adjacent carboxylates, converged, but involved a proton transfer from the N terminus to one carboxylate group (Supplementary Fig. 1B).
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