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Remarkably, Xist transcripts were efficiently spliced after nuclear transfer (Supplementary Figure S4C).
Consistent with this function, Mtch2 at the recipient membrane increased the efficiency of tBid transfer (Supplementary Figure S3).
Similarly, we found no change in predictive power when excluding genomic segments suspected to be derived from horizontal gene transfer (supplementary fig. S12, Supplementary Material online).
This dramatic case seems to result from XGD (Koonin et al. 2001; Koonin 2005) whereby evolutionary histories of apparent orthologs in closely related organisms involve different routes of horizontal gene transfer (supplementary fig. S2, Supplementary Material online).
Muscle and liver did not show changes in markers of immune cell infiltration, inflammation and bacterial translocation into metabolic tissues was not different after microbiota transfer (Supplementary Fig S5B and C).
Moreover, the distribution of male age was normal in the 264 cycles with a fertilization rate ≤20%, indicating no effect of male age on the cycles not reaching transfer (Supplementary data, Fig. S1).
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Finally, 53 deletions were combined in one strain via 28 cycles of deletion-transfer (Supplementary Fig. S2).
Predictions of genomic islands based on abnormal sequence composition (IslandViewer, Langille and Brinkman 2009) also suggested these regions were horizontally transferred (supplementary fig. S6, Supplementary Material online).
These similarities were significant for all COG distributions and for all the KEGG distributions except that of late HT-transfers (supplementary table S1, Supplementary Material online).
In vivo, an incomplete reconstitution with MCs in the uterus and the draining lymph nodes was observed if Lgals1 −/− BMMCs were transferred (Supplementary Figure 11).
As only Tconv are transferred (Supplementary Figure S1a), essentially all Foxp3+ T cells recovered from the recipients are de novo-generated iTregs.
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