Sentence examples for transfer stack from inspiring English sources
The gel was then transferred onto a nylon membrane using an iBLOT DNA transfer stack (Invitrogen) as per the manufacturer's instructions.
In all, 80 μg of tumour extracts was separated in NuPAGE gel (Invitrogen, Camarillo, CA, USA) under reducing conditions and transferred onto nitrocellulose membranes by iBlot gel transfer stack (Invitrogen).
Subsequently, 50 μg of cell extract was analyzed by SDS-PAGE/immunoblotting. Here, NuPAGE 4 12% Bis-Tris Gels (Life Technologies, NP0321) and iBlot Transfer Stack, PVDF Regular (Life Technologies, IB401001) were used.
The transfer stack was then dismantled and the membrane was rinsed with distilled water for 5 minutes before staining with Coomassie blue (0,025% (w/v) Coomassie R-250, 40% (v/v) methanol) for 5 minutes.
For each sample, 40 μg proteins were electrophoresed through 4 12% NuPAGE®NovexBis-Tris SDS PAGE Gel or 3-8% NuPAGE®NovexTris-Acetate SDS PAGE Gel (Invitrogen S.R.L., Life Technologies, Monza, Italy) and electrophoretically transferred to Nitrocellulose Membrane (iBlot® Transfer Stack, Invitrogen S.R.L).
Following electrophoresis, proteins in the gel were transferred onto nitrocellulose membranes (iBlot Gel Transfer Stacks, Invitrogen).
Protein lysates were resolved on denaturing 8 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (iBlot Gel transfer stacks, Invitrogen).
Protein transfers to nitrocellulose membranes were performed using the iBlot™ DryBlotting device (Invitrogen) and iBlot™ Transfer stacks (program P3 for 7 min).
For western blotting, the proteins were transferred onto mini-nitrocellulose membrane using iBlot Gel Transfer Stacks (Invitrogen) following the manufacturer's instructions.
Proteins were separated by loading on Novex NuPAGE 4 12 % Bis-Tris Gels (Life Technologies) and transferring to nitrocellulose with the Novex iBlot Gel Transfer Stacks (Life Technologies).
Proteins were transferred to a nitrocellulose membrane by the iBlot dry blotting system (20 V, 12 min), using iBlot gel transfer stacks.
