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The intention of this study was to identify how specific domains within both isoforms transfer specificity to the individual protein.
Transferring the distal portion of helix 11 between TRiC subunits suffices to transfer specificity for a given substrate motif.
In other words, a lack of transfer specificity during the SABRE process can actually be viewed as being detrimental to catalysis because it corresponds to what is effectively by-product formation.
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Parallel studies with 1-octanol prevented the dye transfer, indicating specificity of GJIC.
A different approach to passively transfer antigen specificity is the transfer of T-cell receptors directed to the nominal Mtb target antigens displayed by major histocompatibility complex (MHC) class I or class II molecules.
A recent and promising intervention is the controlled enlargement of the immune repertoire by transferring tumor specificity.
Here, to provide efficient transfer and increased specificity of transgene expression, we synthesized lentiviral vectors (LVs) with myocardial-specific promoters including: α-myosin heavy chain (α-MHC), myosin light chain (MLC2v), and cardiac troponin T (cTnT).
Second generation designs, which decoupled assembly from DNA strand transfer, afforded the specificity required to identify clinically relevant compounds.
Third, to show that this method is applicable in large scale we use it to transfer DNA-binding specificity data between TFs to enrich the as yet incomplete annotation of DNA binding consensus motifs of TFs.
To determine the transfer efficiency and specificity of adenovirus mediated gene expression driven by the AFpg promoter, cells were transduced with Ad-AFpg-luciferase at various multiplicity of infection (MOI) levels.
Data from Lang et al 44 were available to compare ambulance transport to Injury Severity Scores above versus below 12. Ambulance transfer had low specificity (0.26) but high sensitivity (0.95), opposite to the pattern for hospitalisation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com