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The transfer of cell growth from two-dimensional to three-dimensional culture resulted in changes in ECM-related gene expression consistent with decreasing cell migration and increasing tissue formation.
This non-invasive transfer of cell sheet resulted in shrinkage of the monolayer, enabling the type II cells to regain their cuboidal morphology and specialized characters like Maclura pomifera lectin binding and surfactant protein A (SP-A) expression.
The intercellular transfer of cell membranes and integral membrane proteins has been reported for a wide variety of cells, including cells involved in the immune response, and the passively acquired proteins can alter recipient cell function.
A similar transfer of cell membrane receptors has been observed in many cases and in glioblastomas for example, ExMVs-derived from glioma cells transfer the oncogenic receptor EGFRvIII to neighboring cells and thus increase their transforming potential [36].
In addition to the role of ExMVs in directly stimulating target cells and their role in transfer of cell surface receptors and markers, horizontal transfer of bioactive molecule cargo present inside ExMVs is their most important biological effect.
Trogocytosis involves only the transfer of cell surface antigens without the transfer of DNA or cytoplasm and transdifferentiation is defined as the ability of a differentiated cell to change phenotype to an unrelated cell without undergoing a de-differentiation step [14], [15].
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This transfer of cells into XF assay medium was done just a few hours before the XF assays were run.
Thereby, tight junctions control the paracellular transfer of cells as well as biomolecules, pharmaceuticals, and pathogens58, 59.
In order to examine the effects of migration, we studied pairs of co-cultures and coupled them via transfer of cells between the two patches of each pair.
Based on the MTT test the reduction of the proliferation in 3D scaffolds at 21 and 35 days may indicate also the influence of bulk transfer of cells and therefore an early contact inhibition.
The transfer of cells over-expressing the respective genes may provide a more sustained delivery of the therapeutic molecules and thus be the more economic option.
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