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The same gel was blot-transferred onto polyvinylidene fluoride transfer membrane (GE Healthcare UK Limited, Little Chalfont, Buckinghamshire, UK).
These were subsequently transferred using a nitrocellulose transfer membrane.
The resolved proteins were electroblotted onto the Immobilon-P Transfer Membrane (Millipore).
Proteins in the gel were then transferred to a polyvinylidene difluoride membrane (Immobilon-P transfer membrane, Millipore Corp., MA) using a semidry electrotransfer (15 V constant).
The proteins were then separated through SDS-PAGE and transferred onto the PVDF membrane (Immobion-P Transfer Membrane, Millipore Corp., Billerica, MA, USA).
Proteins were separated on a 12% SDS polyacrylamide gel, and then transferred onto a polyvinylidene difluoride transfer membrane blots (Amersham Hybond P).
Immobilon-P transfer membrane was from Millipore.
Gels were blotted onto Immobilon-P (Millipore) transfer membrane.
After electrophoresis, proteins were transferred to a Trans-Blot nitrocellulose transfer membrane (Bio-Rad).
SDS-PAGE was performed and proteins were transferred onto a PVDF transfer membrane (Amersham Biosciences).
Proteins were transferred to Immobilon-P Transfer Membrane (Millipore Corporation, Bedford, MA).
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