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The 35 kb putative conjugation related region of pKF30-70 contains 40 putative ORFs (from ORF21 to ORF60) between the transfer initiation site (oriT) and a conjugal transfer repressor gene (finO).
Our allele, sy671, is defective in two distinct sub-steps of sperm transfer: initiation and continued transfer.
Of the mutants we isolated, unc-18 sy671), unc-18 sy671 thes work, has defocus in twofdisthist sub-steps of sperm transfer: initiation and continued transfer.
To define the site of action of unc-18 in sperm-transfer behavior, we created a YFP-tagged version of UNC-18 under the regulation of the unc-18 upstream regulatory region (unc-18::UNC-18::YFP), which was able to rescue the sy671 sperm transfer initiation defect (Table 1).
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Two participants required training sessions with an adult to transfer initiations toward peers.
To understand the sperm-transfer initiation defect in sy671 males, we cloned this locus.
As expected, this transgene was able to rescue the sperm-transfer initiation defect in sy671 (Table 1).
In a screen for sperm-transfer-defective mutants, we isolated two mutants, sy671, defective in sperm-transfer initiation, and sy672, defective in the continued transfer of sperm.
When testing animals specifically for sperm-transfer initiation, animals were assayed until the first tonic spicule insertion and transfer was noted.
Because there are no reported markers for the CA neurons, we tested the possible contribution of these neurons to sperm-transfer initiation by assaying lin-39 mutant males.
The gpa-1 UNC-18::YFP transgpa-1 UNC-18 unable to rescue the sperm-transfer initiation defect in sYFP1 (transgene
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