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The transfer frequency for a shaking culture control was low as expected, and mating was not quantifiable in uni-culture biofilms of either H53 or H98 cells (as there is no available source for transfer of the gene required for growth on the selective medium).
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A comparison of transfer frequency was conducted for mixed shaking cultures, colony biofilms and SL-biofilms in chamber slides.
The resulting strains were assessed for conjugative transfer frequency using the drop-plate mating technique described previously (Farrand et al. 2002; Wetzel et al. 2014).
The gene transfer frequency was estimated as the number of transconjugants for each recipient.
In experiments using liquid culture, for more precise calculation of plasmid transfer frequency, the donor cells/recipient cells ratio after cell-mixed culture was determined as follows.
The transfer frequency was calculated as 1.2 × 10-5 and 2 × 10-8 for mupirocin and erythromycin resistance determinants respectively.
The power transfer frequency is 700 kHz.
The transfer frequency values are displayed in Table 2. Overall, the plasmid transfer frequencies were lower than those reported for plasmids found in strains of Clostridium perfringens [25], [26], [27].
The highest transfer frequencies were found for fragments that contained more than two Chi-like sequences, thus, indicating that accumulation of these sequences could create "hot spots" for homologous recombination.
High genes transfer frequencies were inferred for prokaryotic and early eukaryotic evolution.
We analyzed among-strain variation in extinction, successful transfer frequencies, developmental delay, and mortality for the nematodes evolved at N = 1.
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