Sentence examples for transfer constructs from inspiring English sources

For the insertion of the pBeloBAC11 vector into the target position of interest a mini-F transfer constructs was generated using the plasmid pCeu2 [29].

Transfer constructs for the insertion of a genomic duplication into the pBeloBAC11 sequences within ORF22, ORF50, or ORF54 were generated by using the primers E and F, G and H or I and J.

Using corresponding mini-F transfer constructs, the pBeloBAC11 elements were transposed from the unique AvrII site of the VZV US region within the BAC-cloned HJO genome into ORF22, ORF50, or ORF54 of the UL region, into either the coding domain of the duplicated ORF62/71 of the large S repeats, or directly between the genomic termini.

For the generation of the different pHJO variants, the pBelo-HJO-in transfer constructs were digested with I-CeuI and the obtained 7.8 kb fragments were used in the transposition reaction (Fig. 1) to insert 6.4 kb pBeloBAC11 sequences into a novel position and to release the mini-F vector sequences of pHJOFep, resulting in pHJOF22, pHJOF50, pHJOF54, pHJOF62, pHJOF71, or pHJOFpac (Fig. 2 and S1).

The purpose of this study was to evaluate the biomechanical function of the surgically repaired or reconstructed (CC Sling, Rockwood Screw [DePuy Orthopaedics, Warsaw, IN], and Coracoacromial [CA] Ligament Transfer Construct) AC joint after AC joint dislocation.

The transfer construct was engineered within the multiple cloning site (MCS) between the two I-CeuI homing endonuclease restriction sites of plasmid pCeu2.

A KRIT1A-expresssing lentiviral construct was generated from the HIV-derived self-inactivating transfer construct pCCLsin.PPT.PGK.EGFP.Wpre (provided by L. Naldini, HSR-TIGET) by replacing the GFP cassette with the murine KRIT1A cDNA [127].

Approximately 100 300 ng of a 7.8 kb mini-F transfer construct derived by I-CeuI-digestion of a corresponding pBelo-HJO-in plasmid were introduced by electroporation into GS1783 to insert the 6.4 kb pBeloBAC11 derivate via the 0.7 kb homologous sequences into the target position of interest in pHJOFep (Fig. 1C).

First, the mini-F transfer construct was released from the pCeu2 vector backbone and used to insert the 6.4 kb pBeloBAC11 derivate via Red-mediated recombination of the 0.7 kb flanking homologous viral sequences into the target position of interest in pHJOFep (Fig. 1C).

This transfer construct consisted of a 6.4 kb modified pBeloBAC11 vector without cos and loxP sequences flanked by 0.7 kb viral sequences homologous to regions located at either site of the target position of interest (Fig. 1B and 1C, orange and yellow elements).

In the mini-F sequence transposition reaction, the pBeloBAC11 vector was inserted into the target position of interest using the prepared transfer construct and the aphAI-I-SceI-mini-F cassette was excised via two successively induced Red recombinations (Fig. 1C).