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Transfer cell morphology is one of the common structural specializations observed in suspensor cells.
Subsequent procedures included mammalian somatic cell nuclear transfer, cell fusion, induction of pluripotency by ectopic gene expression, and direct reprogramming.
Biological therapies for treatment of disc degeneration include protein injection, gene transfer, cell therapy and tissue engineering.
The specimen was then mounted onto an anaerobic Gatan environmental transfer cell and transferred into the TEM without being exposed to the atmosphere.
Proteins were separated by SDS-PAGE followed by Western blotting, using a Mini-Trans Blot Electrophoretic Transfer Cell (BioRad).
Proteins were electrophoretically transferred to nitrocellulose membranes by means of a Mini transblot electrophoretic transfer cell (Bio-Rad).
Gels were transferred to polyvinylidene fluoride (PVDF) membrane at 15V for 1 hour on a Trans-Blot Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA).
Proteins were transferred to a PVDF membrane (GE Healthcare) using a semidry transfer cell system (Biorad), and blocked by immersion in 5% skimmed milk in PBS.
For Western blotting, the gel was subjected to horizontal blotting onto a nitrocellulose membrane using a PAGEgel transfer cell (180 mA constant, 100 min).
For Western blot, proteins were blotted after SDS-PAGE onto nitrocellulose membranes (Porablot NCL, Macherey-Nagel) using a Trans Blot SD transfer cell (Bio-Rad).
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