Sentence examples for transfer buffer for from inspiring English sources

For Western blotting, wash the gel in transfer buffer for 20 min.

Proteins were transferred onto a PVDF membrane (Millipore Corp ,Bedford, MA) in transfer buffer for 1 hour at 300 mA.

Gels were equilibrated in transfer buffer for 15 min.

Proteins were separated on SDS PAGE gels and transferred to a polyvinylidene difluoride membrane in a wet tank using transfer buffer for 1.5 h [ 78].

#Equilibrate gel in transfer buffer for 15 min. #Meanwhile, cut membrane and 4 pieces 3 MM filter paper to size of gel.

Subsequently, the gel was incubated with 0.1% SDS in transfer buffer for 10 min at room temperature (RT) and transferred to a polyvinylidene difluoride membrane.

Gels were blotted by semi wet transfer (Invitrogen × Cell II Blot Module) with NuPAGE Transfer Buffer, 10% (for one gel) or 20% methanol (for two gels) on polyvinylidene difluoride transfer membrane (Immobilon-P, Invitrogen) at constant 30 V for 1 h.

Protein was transferred to a nitrocellulose membrane using NuPAGE transfer buffer (Invitrogen) for two hours and blotted as described below.

Separated proteins were transferred onto a nitrocellulose membrane using a wet blotter in the Genie Transfer buffer (pH 8.4) for 90 min at 250 mA.

Separated proteins were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Darmstadt, Germany) using a wet blotter in the Genie Transfer Buffer (pH 8.4) for 90 min at a constant current of 250 mA.

After transfer on a nitrocellulose membrane in Nupage transfer buffer (25 V for 1.5 hr), we blocked nonspecific interactions by incubating the blot overnight at 4°C in PBS supplemented with 5% nonfat dry milk and 1% BSA.