Sentence examples for transfer buffer from inspiring English sources

Proteins were electro-transferred onto nitrocellulose membranes in transfer buffer.

Proteins were electro-transferred to NC membrane in transfer buffer at 100 V, 4 °C for 1 h.

Hind feet were dissected and immersed in transfer buffer (Hank's buffered salt solution supplemented with 10 mM HEPES, pH 7.4).

A transfer buffer is set to enable conversion between segments and connect each independent zone.

Briefly, gels containing samples were transferred to a PVDF membrane for 2 h at 800 mA in transfer buffer [48 mM Tris, 39 mM glycine, and 15% methanol] at 4°C.

Proteins were then transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA) for 40 min at 20 V in a pH 10, MeOH free transfer buffer (5.82% tris-base, 2.93% glycine).

Protein was then transferred to 0.45 µm pore nitrocellulose membranes (Life Technologies) in 1× Western transfer buffer (Life Technologies) at a constant 30 V for 1 h using an XCell II Blot module (Life Technologies).

For Western blotting, wash the gel in transfer buffer for 20 min.

Because of the large size of HCNCp, transfer buffer contained 20% MeOH/0.1% SDS.

The transfer buffer consisted of 1% (v/v) SDS to ensure complete transfer of proteins.

Proteins were transferred onto nitrocellulose membranes (Amersham Biosciences) in transfer buffer (25 mM Tris, 192 mM glycine, 20% ethanol).