Sentence examples for transfer assay with from inspiring English sources

The resulting samples were then subjected to the label transfer assay with Ssa2p.

(C ) Wild-type (WT) and mutant forms (mut1 and mut2) of human tRNA-LeuAAG and tRNA-ArgUCG were subjected to the label transfer assay with Ssa2p.

The residues around K125 (DNKQQ) are individually or collectively mutated to Ala or Glu and product formation at 15 s in pulse-chase fluorescent Ub transfer assay with Rsp5WW1-3-HECT ishownwn.

We then conducted a transfer assay with B4GAT1dTM as the enzyme source, UDP-GlcA as the sugar donor and fluorescently labeled β-xyloside (4-methylumbelliferyl-β-D-xyloside, Xyl-β-MU) as the acceptor.

(B ) Label transfer assays with full-length Ssa-His6 fusions or GST-fusions with partial Ssa proteins.

(A ) Label transfer assays with P-labeled tRNA-ProUGG were performed in the presence of various concentrations of RCMLA.

(B ) Label transfer assays with P-labeled mature tRNA-ProUGG were performed in the presence of various concentrations of ATP.

(A ) In vitro-transcribed tRNA-ProUGG molecules shown in the lower panel (full-length [full], CCA-less [-CCA], and precursor [pre]) were subjected to label transfer assays with recombinant Ssa1p or Ssa2p in the absence or presence of 2.5 mM ATP. P-labeled Ssa proteins are indicated by an arrowhead.

To determine whether the mitochondrial transfer function of the intermediate vesicles was affected in endosome-depleted cells, the transfer assay was performed with the cytosolic fraction from normal or EEA1-KD cells transfected with GFP RNA for 2 h (Fig. 6C).

The cell transfer assay was repeated with labelled P-gp.

In a bulk transfer assay, OMsfGFP and OMmCherry were transferred with similar efficiencies, showing that OMsfGFP could be used in a single cell assay.