Sentence examples for transfer and detected from inspiring English sources

Proteins were electrotransferred onto a nitrocellulose membrane using wet transfer and detected by specific antibodies combined with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit, goat anti-mouse, Bio-Rad, Hercules, CA, USA).

Multiple gels were run to process all samples from a single experiment, but were transferred and detected in parallel and imaged simultaneously.

The beads were washed with 50 mL of buffer C, followed by 50 mL of buffer D. Rhodopsin was eluted with buffer E. The opsin expression and the purity of the purified rhodopsin were analyzed by SDS-PAGE and the expressed proteins were electrotransferred onto polyvinylidene fluoride transfer membrane and detected using the rho-1D4 monoclonal antibody using western blot analysis.

Proteins were transferred onto Immobilon-P transfer membranes (Millipore) and detected with anti-gD polyclonal antibody, anti-HA monoclonal antibody (Sigma) or anti-human IgG antibody (Jackson Immunoresearch, West Grove, PA, USA).

Proteins were eluted from the beads by heating at 95°C in 1× Laemmli buffer, resolved on 4 20% gradient gels (BioRad), transferred to nitrocellulose and detected by western blot using mouse monoclonal anti-HA or anti-myc antibodies, HRP-conjugated goat anti-mouse secondary antibodies (Jackson Immunoresearch) and chemiluminescent detection (Pierce).

Biotinylated samples were run on SDS-PAGE, transferred to a nylon membrane, and detected with nucleic acid detection kit (Thermo).

The biotinylation mixture was resolved on 12% SDS-PAGE, transferred onto nitrocellulose membrane and detected by streptavidin HRP.

Proteins were transferred onto PVDF membranes and detected by primary antibodies (mouse, α-his and α-flag) followed by the secondary antibody rabbit, α-mouse conjugated to alkaline phosphatase.

Cell extracts were heated to 95°C for 5 min, run out on a 10% polyacrylamide gel, transferred to PVDF membrane, and detected using the FLAG M2 antibody (Sigma-Aldrich, St . Louis MO).

60ug were loaded on 12% SDS polyacrylamide gel and transferred to a PVDF membrane and detected with anti-HuC/HuD monoclonal antibody (1∶1000, Invitrogen).

The gel was transferred to a nylon membrane and detected using streptavidin-HRP and chemiluminescent substrate.